yhbJ [2020-04-14 19:03:57]

putative efflux system component

locus: BSU_09000

pI: 4.37

mw: 23.47 kDa

protein length: 221 aa

gene length: 666 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 977,069 977,734

The protein

Protein family

• [SW|Membrane fusion protein (MFP) (TC 8.A.1) family] (according to UniProt)

Structure

• [PDB|4TKO] (from Aquifex aeolicus, corresponds to aa 74 ... 202, 35% identity)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|EDDD61CF725449E57C72F63D378F6CD1FAC49ADA|yhbI]-[gene|211C58A92BA2A06A34B1F73BDB68A0F581B942C2|yhbJ]-[gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]-[gene|7797B0B66922D51B9D142DD2E5857836E47F015B|yhcB]-[gene|95EED3A91483502CA78067421F678B1EDB965B18|yhcC]
  description: [Pubmed|22383849]

  additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 4 to 41 min) [PubMed|21815947]

Biological materials

Mutant

• MGNA-B470 (yhbJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1469 NBRP B. subtilis, Japan]
• BKE09000 ([gene|211C58A92BA2A06A34B1F73BDB68A0F581B942C2|yhbJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGCGGAAAGCTGGTG, downstream forward: _UP4_TAAAACAGATCAGTCATCAA
• BKK09000 ([gene|211C58A92BA2A06A34B1F73BDB68A0F581B942C2|yhbJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGCGGAAAGCTGGTG, downstream forward: _UP4_TAAAACAGATCAGTCATCAA

References

• 21815947